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Open Access Original article

Degradative actions of microbial xylanolytic activities on hemicelluloses from rhizome of Arundo donax

Licia Lama1, Annabella Tramice1, Ilaria Finore1, Gianluca Anzelmo1, Valeria Calandrelli1, Eduardo Pagnotta1, Giuseppina Tommonaro1, Annarita Poli1, Paola Di Donato12, Barbara Nicolaus1, Massimo Fagnano3, Mauro Mori3, Adriana Impagliazzo3 and Antonio Trincone1*

Author Affiliations

1 Institute of Biomolecular Chemistry ICB-CNR, Consiglio Nazionale delle Ricerche, Via Campi Flegrei 34, Pozzuoli, 80072, Napoli, Italy

2 Department of Environmental Sciences, University of Naples “Parthenope”, Centro Direzionale, Isola C4, Naples, 80143, Italy

3 Department of Agriculture, Naples University Federico II, Via Università 100, Portici, 80055, Naples, Italy

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AMB Express 2014, 4:55  doi:10.1186/s13568-014-0055-6

Published: 9 July 2014

Abstract

Polysaccharidases from extremophiles are remarkable for specific action, resistance to different reaction conditions and other biotechnologically interesting features. In this article the action of crude extracts of thermophilic microorganisms (Thermotoga neapolitana, Geobacillus thermantarcticus and Thermoanaerobacterium thermostercoris) is studied using as substrate hemicellulose from one of the most interesting biomass crops, the giant reed (Arundo donax L.). This biomass can be cultivated without competition and a huge amount of rhizomes remains in the soil at the end of cropping cycle (10–15 years) representing a further source of useful molecules. Optimization of the procedure for preparation of the hemicellulose fraction from rhizomes of Arundo donax, is studied. Polysaccharidases from crude extracts of thermophilic microorganisms revealed to be suitable for total degradative action and/or production of small useful oligosaccharides from hemicelluloses from A. donax. Xylobiose and interesting tetra- and pentasaccharide are obtained by enzymatic action in different conditions. Convenient amount of raw material was processed per mg of crude enzymes. Raw hemicelluloses and pretreated material show antioxidant activity unlike isolated tetra- and pentasaccharide. The body of results suggest that rhizomes represent a useful raw material for the production of valuable industrial products, thus allowing to increase the economic efficiency of A. donax cultivation.

Keywords:
Biorefinery; Xylan; Thermophilic enzymes; Xylanases; Xylooligosaccharides; Arundo donax