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Open Access Highly Accessed Original article

Lactic acid production by Enteroccocus faecium in liquefied sago starch

Cirilo Nolasco-Hipolito13*, Octavio Carvajal Zarrabal2, Rubena Malfia Kamaldin3, Ling Teck-Yee1, Samuel Lihan1, Kopli Bin Bujang1 and Youji Nitta4

Author Affiliations

1 Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300, Kota Samarahan, Sarawak, Malaysia

2 Biochemical and Nutrition Chemistry Area, University of Veracruz, SS Juan Pablo II s/n, Boca del Río, CP 94294, Veracruz, Mexico

3 SpeCorp, Sdn Bhd, Level 2 Block 218 KNLD., Jln Tun Ahmad Zaidi Adruce, Sarawak, Kuching, 93200, Malaysia

4 The College of Agriculture, Ibaraki University, 3-21-1, Chuuo, Ami, Inashiki, Ibaraki, 300-0393, Japan

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AMB Express 2012, 2:53  doi:10.1186/2191-0855-2-53

Published: 28 September 2012

Abstract

Enterococcus faecium No. 78 (PNCM-BIOTECH 10375) isolated from puto, a type of fermented rice in the Philippines was used to produce lactic acid in repeated batch fermentation mode. Enzymatically liquefied sago starch was used as the sole carbon source, since sago (Metroxylon spp) is a sustainable crop for industrial exploitation. Liquefied sago starch was inoculated with E. faecium to perform the saccharification and fermentation processes simultaneously. Results demonstrated that E. faecium was reused for 11 fermentation cycles with an average lactic acid yield of 36.3 ± 4.71 g/l. The lactic acid production was superior to that of simple batch mode and continuous fermentation in terms of lactic acid concentration. An un-dissociated lactic acid concentration of 1.15 mM affected the productivity of the cells. Work is in progress to maintain and increase the usability of the cells over higher fermentation cycles.

Keywords:
Enteroccus faecium; Lactic acid; Repeated batch fermentation; Liquefied sago starch; Cell reuse