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Purification and characterization of hydroquinone dioxygenase from Sphingomonas sp. strain TTNP3

Boris A Kolvenbach1,2*, Markus Lenz1, Dirk Benndorf3, Erdmann Rapp4, Jan Fousek5,6, Cestmir Vlcek5,6, Andreas Schäffer2, Frédéric LP Gabriel7, Hans-Peter E Kohler8 and Philippe FX Corvini1,9

1 Institute for Ecopreneurship, School of Life Sciences, University of Applied Sciences Northwestern Switzerland, Muttenz, Switzerland

2 Institute for Environmental Research, Rheinisch-Westfälische Technische Hochschule, Aachen, Germany

3 Bioprocess Engineering, Otto von Guericke University, Magdeburg, Germany

4 Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany

5 Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic

6 Centre for Applied Genomics, Prague, Czech Republic

7 Institute of Clinical Chemistry and Laboratory Medicine, University of Rostock, Rostock, Germany

8 Swiss Federal Institute of Aquatic Science and Technology, Dübendorf, Switzerland

9 School of the Environment, Nanjing University, Nanjing, China

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AMB Express 2011, 1:8 doi:10.1186/2191-0855-1-8

Published: 27 May 2011

Additional files

Additional file 1:

Proposed fragmentation pattern for trimethylsilylated 4-hydroxymuconic semialdehyde and its methylated analogon. 1, proposed GC-MS fragmentation pattern of 4-hydroxymuconic semialdehyde; 2, proposed GC-MS fragmentation pattern of the ring-cleavage product of 2-methyl-hydroquinone with cleavage sites between the ring bonds C(1)-C(6), C(3)-C(4) and C(5)-C(6), respectively; 3, proposed GC-MS fragmentation pattern of the ring-cleavage product of 2-methyl-hydroquinone with cleavage sites between the ring bonds C(1)-C(2); 4, GC-MS fragmentation pattern of 2-hydroxy-6-(2-hydroxyphenyl)-6oxo-2,4-hexadienoic acid (Kohler et al. 1993).

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Additional file 2:

Multiple sequence alignment performed by ClustalW 2 of the sequence of HqdA with sequences retrieved by BLAST search. Shown is the original multiple sequence alignment from which Figure 4A has been rendered.

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Additional file 3:

Multiple sequence alignment performed by ClustalW 2 of the sequence of HqdB with sequences retrieved by BLAST search. Shown is the original multiple sequence alignment from which Figure 4B has been rendered.

Format: PDF Size: 75KB Download file

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Open Data