Figure 1.

Alignment of the deduced amino acid sequence of CphE_al with other known and predicted cyanophycinases and PepE. The potential signal peptides of the extracellular CGPases are underlined. Only the residues in the vicinity of the catalytic site are shown for CphB and PepE. The proposed catalytic residues (GXSXG, H and E) are shaded light gray. Residues proposed to be involved in substrate binding (Law et al. 2009) are shaded dark gray. For sequence alignment, the CLUSTAW was used. CphE_al from P. alcaligenes DIP1; Cyanophycinase from P. mendocina; CphE_pa from P. anguilliseptica BI; Cyanophycinase from Synechocystis PCC6803; PepE from Salmonella typhimurium. Numbering for the CphE_al protein is given at the end of the line.