Treatment of particulate cell debris with detergents, chelating agents and chaotropic reagents. Recovered activities of supernatants before (100% relative activity) and after incubation of particulate cell debris (obtained from a 56 h-cultivation) with selected solubilising detergents in increasing concentrations for 30 minutes, monitored by oxidation of 2,6-DMP. Error bars refer to standard deviation by means of four replicates.
Herter et al. AMB Express 2011 1:14 doi:10.1186/2191-0855-1-14